TY - JOUR AU - Bertin, Larissa Dragonetti AU - Pires-Oliveira, Deise Aparecida Almeida AU - Oliveira, Priscila Daniela AU - Serpeloni, Juliana Almeida AU - Szezerbaty, Stheace Kelly Fernandes AU - Santos, João Paulo Manfre AU - Oliveira, Rodrigo Franco PY - 2015/11/18 Y2 - 2024/03/29 TI - The influence of ultrasonic radiation of low intensity in cultured fibroblast cells JF - Manual Therapy, Posturology & Rehabilitation Journal JA - mtprehabjournal VL - 0 IS - 0 SE - Research articles DO - 10.17784/mtprehabJournal.2015.13.306 UR - https://submission-mtprehabjournal.com/revista/article/view/1097 SP - 1-5 AB - <p>Introduction: In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cell<br>function (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This study<br>will analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data from<br>each exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The control<br>group (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work),<br>0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performed<br>through optical microscopy, MTT method 3 - (4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation times<br>of 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulation<br>process and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process,<br>neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simple<br>and informative model on the significant aspects of the use of physical therapy in vivo system.</p> ER -